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  1. Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster - Prohealth
  2. Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster
  3. Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster
  4. Antibacterial and antifungal activities of Cistus incanus and C. monspeliensis leaf extracts

Cistus libanotis, Cistus villosus, Cistus villosus L., Cistus monspeliensis, Cistus ladanifer, Cistus populifolius, Cis- tus salviifolius, Cistus parviflorus, and Cistus. The growing popularity of the Cistus species of herbs is mainly due to its tis, C. villosus and C. monspeliensis, C. ladanifer and C. populifolius. leaves of two Cistus species; C. monspeliensis and C. salvifolius were Tunisian Cistus libanotis, Cistus Monspeliensis and Cistus villosus.

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Cistus Villosus Pdf

PDF | The family Cistaceae (Angiosperm, Malvales) consists of 8 genera and species, with 5 genera native to the Mediterranean area (Cistus, Fumara. Tunisians' Cistus monspeliensis, Cistus libanotis and. Cistus villosus rock rose species and indicated diterpenes compounds as the main constituents of the. Authors noticed that among eight plant extracts, only methanol extracts of Cistus villosus, Ceratonia siliqua, and Halimiumum bellatum exhibited strong.

Cistus is a genus of beautiful flowering plants that are native to Mediterranean regions of Southern Europe and North Africa. Commonly referred to as Rockroses, flowers in the Cistus genus bloom in lovely colors ranging from pure white to bright pink. Just looking at one of these plants, one would not suspect that it possesses very powerful medicinal properties. In traditional herbal medicine , the leaves of Cistus have been used in the treatment of skin and inflammatory diseases Hudson, Recent scientific research has confirmed the validity of this traditional herbal knowledge through studies that have demonstrated that Cistus leaf extractives have powerful antibacterial, antifungal, antiviral, and biofilm-breaking qualities Rebensburg et al, The many benefits of Cistus have made it a staple in my own treatment protocol for Lyme disease and Lyme co-infections. Read on to find out why I am such a huge fan of this beautiful little plant! Antibacterial and Antifungal Benefits of Cistus Plants in the Cistus genus exert a number of powerful antibacterial and antifungal properties against pathogens that can cause serious human health problems. Cistus incanus one particular species of Cistus has demonstrated antibacterial effects against Streptococcus mucans, a gram-positive bacterium found in the mouth that can contribute to tooth decay Wittpahl, Cistus villosus and Cistus monspeliensis, two species native to Morocco, have been shown to be antibacterial against Staphylococcus aureus, which is the bacterium responsible for causing Staph infections. Antibiotic-resistant Staph infections are on the rise, so natural antibacterial substances that can address this type of infection are highly valuable! Candida overgrowth can wreak havoc on the digestive tract, and Aspergillus infections are toxic to the immune system and the respiratory system Sharma, The fact that Cistus can address both of these types of fungi is great news for those of us who are struggling with fungal illnesses, including toxic mold exposure. These antifungal properties could make Cistus an important part of healing protocols for people with mold-induced illness. I personally have struggled with a mold-related illness after living in an apartment that had a water leak and subsequent mold problem.

More recently, a large body of studies is evidencing the ability of these compounds to modulate uncontrolled proliferation pathways or protooncogen expression [ 22 ]. Therefore, it is certainly plausible that the antiproliferative activity against A cell line of the hexane extract of Cistus monspeliensis compounds could be related to their radical scavenging activity too.

Phenolic compounds have been traditionally associated to biological activities such as antioxidant, antimicrobial or cytotoxic. A recent study on the anticancer activity of several tea extracts with high polyphenolic content has reported IC50 values within the range 0. Therefore, the concentration ranges of C. Vitamin E acts as a peroxyl radical scavenger, preventing the propagation of free radicals in tissues, by reacting with them to form a tocopheryl radical which will then be oxidized by a hydrogen donor such as Vitamin C and thus return to its reduced state [ 24 ].

As it is fat soluble, it is incorporated into cell membranes and protects them from oxidative damage. The cancer preventive properties of vitamin E were firstly suspected when some studies showed that people in the Mediterranean area who consume diets enriched in vitamin E displayed a lower risk of colon cancer than people in Northern Europe and the U. More recently, the Melbourne Colorectal Cancer Study showed that dietary vitamins E and C were protective for both colon and rectal cancer, and that for both vitamins there was a dose—response effect of increasing protection [ 27 ].

Another clinical study supported a preventive effect of vitamin E in the development of prostate cancer.

Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster - Prohealth

More recently it has been demonstrated that Vitamin E also protects lipids and prevents the oxidation of polyunsaturated fatty acids [ 29 ]. Experimental studies also suggested detrimental effects of omega-6 polyunsaturated fatty acids PUFA , and beneficial effects of omega-3 PUFAs on mammary carcinogenesis, possibly due to the interaction with antioxidants. Significant interactions were also found between omega-6 and long-chain omega-3 PUFAs, with breast cancer risk inversely related to long-chain omega-3 PUFAs [ 30 ].

In this light, it is interesting to note that the hexane extract of C. In this sense, C. Experimental Plant material Leaves of C.

Extraction The plant material was dried under shade and gross powdered prior to extraction. Helium was the carrier gas 1 mL min Identification of components Most constituents were identified by gas chromatography by comparison of their retention indices LRI with either those of the literature [ 31 , 32 ] or with those of authentic compounds available in our laboratories.

The retention indices were determined by GC-FID mode in relation to a homologous series of n-alkanes C8-C28 under the same operating conditions.

Further identification was made by comparison of their mass spectra on both columns with either those stored in NIST 02 and Wiley libraries or with mass spectra from the literature [ 32 , 33 ] and our home made library. Component relative concentrations were calculated based on GC-FID peak areas without using correction factors.

Cell lines J MTT antiproliferative assay Cells J Al, A, and MCF-7 were harvested and suspended in complete culture media. In some experiments, serial dilutions of 6-MP, as reference drug, were added.

The cell viability was assessed through an MTT conversion assay Bianco et al.

Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster

All the experiments were carried out in triplicates and two independent experiments were performed for each test sample. Conclusion The chemical composition of the hexane extracts from the three Cistus species can be useful in the chemosystematics of this complex genus. The antiproliferative activity of the Cistaceae hexane extracts observed for the first time in this study against human melanoma cell line A deserve further investigations in order to determine the compounds, or their combinations, which are the main responsible for antiproliferative activity and its potential mechanism.

Findings and description of additional material Plant material and extracts of the plants are available MB. Competing interests The authors declare that they have no competing interests.

MEK Collected and identified the plant material and drafted the manuscript. GA performed the MTT antiproliferative assay and drafted the manuscript. SM performed the MTT antiproliferative assay and drafted the manuscript.

A1, human melanoma cells, A, and Diterpenes 2. In through the MTT conversion assay [17]. In Table 2 we C. Hydrocarbons Vitamin E was present Monoterpenes, oxygen- monspeliensis and C. A1 macrophages, A human melanoma cells and present in quite low amount.

The peculiar characteristic MCF-7 breast cancer cells at 72 h of the composition of the extract of C. Cistus libanotis N. Fatty acids It is noteworthy the good quantity of Cistus villosus N.

Few reports are available in literature about the chemical N. The IC50 value is the ition of a hexane extract of leaves of C. Both extracts obtained from C. More recently it has been demonstrated that timated.

A pronounced growth inhibition was showed Vitamin E also protects lipids and prevents the oxidation by C. Significant interactions were also found between Natural extracts have been previously reported as a omega-6 and long-chain omega-3 PUFAs, with breast potential source of antiproliferative compounds []. In this light, it is interesting to note that the and tumor-inhibitory effect associated to some dietary hexane extract of C.

Cistus: A Natural Antibiotic, Antiviral and Biofilm Buster

More recently, a large body of studies is evidencing acid A recent study on the anticancer activity of Experimental several tea extracts with high polyphenolic content has Plant material reported IC50 values within the range 0. The plant material was dried under shade and gross Vitamin E acts as a peroxyl radical scavenger, preventing powdered prior to extraction. The powdered leaf 30 g the propagation of free radicals in tissues, by reacting was extracted three times with mL of hexane for 3 with them to form a tocopheryl radical which will then days, than the extracts were filtered through a filter be oxidized by a hydrogen donor such as Vitamin C paper, after that the extracts were concentrated by rota- and thus return to its reduced state [24].

Column temperature was initially Europe and the U. An- in the splitless mode with a 1 minute purge-off due to other clinical study supported a preventive effect of the small amount of oil partially utilized for biological vitamin E in the development of prostate cancer. This tests. Helium was the carrier gas 1 mL min The optical density OD of each well was plier voltage energy V.

All the experiments Identification of components were carried out in triplicates and two independent ex- Most constituents were identified by gas chromatog- periments were performed for each test sample. The retention indices were determined by GC-FID mode in relation to a homologous series of n-alkanes C8-C28 Conclusion under the same operating conditions. Further identifica- The chemical composition of the hexane extracts from the tion was made by comparison of their mass spectra on three Cistus species can be useful in the chemosystematics both columns with either those stored in NIST 02 and of this complex genus.

The antiproliferative activity of the Wiley libraries or with mass spectra from the litera- Cistaceae hexane extracts observed for the first time in ture [32,33] and our home made library. Component this study against human melanoma cell line A de- relative concentrations were calculated based on GC- serve further investigations in order to determine the FID peak areas without using correction factors.

GA performed the MTT antiproliferative assay and drafted the manuscript. SM performed the MTT antiproliferative assay and drafted the manuscript. MB prepared the extracts, Cells J Al, A, and MCF-7 were harvested and identified the components and drafted the manuscript. All authors read and suspended in complete culture media.

Approximately approved the final manuscript. Montesano, , Naples, Italy. BMC Cancer , J Agric Food Chem , — Cistaceae seed banks and its influence on techniques for quantifying Plant Ecol , — Free Radic Biol Med , — Berrino F, Muti P: Mediterranean diet and cancer. Mol Phylogenet Evol , — Khlat M: Cancer in Mediterranean migrants—based on studies in France 3.

Antibacterial and antifungal activities of Cistus incanus and C. monspeliensis leaf extracts

Cancer Causes Contr , — Ind Crop Prod , — Kune G, Watson L: Colorectal cancer protective effects and the dietary 4. Phytother Res lycopene. Nutr Cancer , — J Natl Cancer Inst bioassayguided procedures. J Ethnopharmacol , — California: Cengage Learning; Int J Cancer , — Essential oils and hexane extracts from Leaves and fruits of Cistus

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